Search results for " high-performance liquid chromatography"

showing 3 items of 3 documents

Influence of roasting and different brewing processes on ochratoxin A content in coffee determined by high-performance liquid chromatography-fluoresc…

2008

A rapid and reliable procedure has been developed for the determination of ochratoxin A (OTA) in green and roasted coffee. The method consists of extraction of the sample with methanol-5% aqueous sodium hydrogen carbonate/1% PEG8000 (20:80), followed by immunoaffinity column (IAC) clean-up and, finally, high-performance liquid chromatography (HPLC) determination with fluorimetric detection. Mean recoveries for green and roasted coffee spiked at different levels ranging from 94 and 105% were obtained. The limit of determination (S/N = 3) was 0.032 ng g(-1) and the precision (within-laboratory relative standard deviation) was 6%. The method described has been used to assess the influence of r…

Ochratoxin AHot TemperatureSettore CHIM/10 - Chimica Degli AlimentiFood HandlingHealth Toxicology and MutagenesiscoffeeFood ContaminationToxicologyHigh-performance liquid chromatographybrewing methodchemistry.chemical_compoundHplc fldMycotoxinBrewing methods; Coffee; High-performance liquid chromatography-fluorescence detection (HPLC-FLD); Ochratoxin AOchratoxinChromatography High Pressure LiquidRoastingBrewing methodsChromatographybusiness.industryPublic Health Environmental and Occupational HealthGeneral ChemistryGeneral MedicineMycotoxinsFluorescenceOchratoxinshigh-performance liquid chromatography-fluorescence detection (HPLC-FLD)chemistryCarcinogensBrewingbusinessochratoxin AFood Science
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Wild

2021

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0106 biological sciencesGermplasmPhytochemistryTPCC total phenolic contentElderberry flowerISSR inter-simple sequence repeatElderberry fruitIC50 the half maximal inhibitory concentrationBerrySambucus nigra01 natural sciencesArticleTFC total flavonoid contentDW dry weightSARS-CoV2 severe acute respiratory syndrome coronavirus 2Rutinchemistry.chemical_compoundBotanyELISA enzyme linked immunosorbent assayCultivarAntiviralComputingMethodologies_COMPUTERGRAPHICSGenetic diversityACE2 angiotensin converting enzyme 2biology010405 organic chemistryPVPP polyvinylpyrrolidoneSambucus nigra L.biology.organism_classification0104 chemical scienceschemistryHPLC high-performance liquid chromatographyTEAC trolox equivalent anti-radical capacityGene poolAgronomy and Crop Science010606 plant biology & botanyBinding domainIndustrial crops and products
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Age-dependent regulation of antioxidant genes by p38α MAPK in the liver

2018

p38α is a redox sensitive MAPK activated by pro-inflammatory cytokines and environmental, genotoxic and endoplasmic reticulum stresses. The aim of this work was to assess whether p38α controls the antioxidant defense in the liver, and if so, to elucidate the mechanism(s) involved and the age-related changes. For this purpose, we used liver-specific p38α-deficient mice at two different ages: young-mice (4 months-old) and old-mice (24 months-old). The liver of young p38α knock-out mice exhibited a decrease in GSH levels and an increase in GSSG/GSH ratio and malondialdehyde levels. However, old mice deficient in p38α had higher hepatic GSH levels and lower GSSG/GSH ratio than young p38α knock-…

ROS Reactive oxygen species;RSK1 Ribosomal S6 kinase10301 basic medicineMAPK/ERK pathwayAgingHPLC High-performance liquid chromatographyAntioxidantmedicine.medical_treatmentTBP TATA-binding proteinClinical BiochemistryDEN Diethyl nitrosamine;MKP-1 MAPK phosphatase-1IκB kinaseGCLc Glutamate cysteine ligase catalytic subunitp38 Mitogen-Activated Protein KinasesG6PDH Glucose-6-phosphate dehydrogenaseBiochemistryAntioxidantsMicechemistry.chemical_compoundSuperoxide Dismutase-1Akt Protein kinase B0302 clinical medicineNrf2 Nuclear factor erythroid 2-related factor-2IL InterleukinSOD1 Cu/Zn-superoxide dismutaselcsh:QH301-705.5Mice KnockoutMK2 MAP-activated protein kinase 2;PGC-1α Peroxisome proliferator-activated receptor gamma coactivator 1-alphachemistry.chemical_classificationlcsh:R5-920Trx ThioredoxinGlutathione DisulfideTNF-α Tumor necrosis factor-alphabiologyLPS Lipopolysaccharide;GSSG Oxidized glutathione;MEF Mouse embryonic fibroblastsNF-kappa BGstm1 Glutathione S-transferase mu 1CatalaseEndoplasmic Reticulum StressGlutathioneLiverGSH Reduced glutathione;Catalase030220 oncology & carcinogenesisJNK c-Jun N-terminal kinaselcsh:Medicine (General)Research Papermedicine.medical_specialtyNF-E2-Related Factor 2Glutamate-Cysteine LigaseMKK MAPK kinaseAP-1 Activator protein-1IKK IƙB KinaseGene Expression Regulation EnzymologicSuperoxide dismutase03 medical and health sciencesInternal medicineGlutamate cysteine ligaseEGFR Epidermal growth factor receptormedicineAnimalsNuclear factor ƙBAnd catalaseChIP Chromatin immunoprecipitation;Protein kinase BNF-ƙB Nuclear factor kappa BSuperoxide DismutaseSuperoxide dismutase 1Superoxide dismutase 2Organic ChemistryGlutathioneASK1 Apoptosis signal-regulating kinase 1ATF2 activating transcription factor 2;030104 developmental biologyEndocrinologyEnzymeHsp Heat shock proteinlcsh:Biology (General)chemistrybiology.proteinSOD2 Mn-superoxide dismutaseMAPK mitogen activated protein kinaseNEM N-ethyl maleimide;Redox Biology
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